Advanced Cell Diagnostics

Provider of the most advanced
RNA in situ hybridization technology
A Novel RNA In Situ Hybridization Assay for the Long Noncoding RNA SChLAP1 Predicts Poor Clinical Outcome After Radical Prostatectomy in Clinically Localized Prostate Cancer, Mehra R, Shi Y, Udager AM, Prensner JR, Sahu A, Iyer MK, Siddiqui J, Cao X, Wei J, Jiang H, Feng FY, Chinnaiyan AM., Neoplasia, 2014 December 11 --- Do high-risk human papillomaviruses cause oral cavity squamous cell carcinoma?, Mirghani H, Amen F, Moreau F, Lacau St Guily J., Oral Oncology, 2014 December 05 --- Piezo2 is the major transducer of mechanical forces for touch sensation in mice, Ranade SS, Woo SH, Dubin AE, Moshourab RA, Wetzel C, Petrus M, Mathur J, Bégay V, Coste B, Mainquist J, Wilson AJ, Francisco AG, Reddy K, Qiu Z, Wood JN, Lewin GR, Patapoutian A., Nature, 2014 December 04 --- Aberrant epithelial GREM1 expression initiates colonic tumorigenesis from cells outside the stem cell niche., Davis H, Irshad S, Bansal M, Rafferty H, Boitsova T, Bardella C, Jaeger E, Lewis A, Freeman-Mills L, Giner FC, Rodenas-Cuadrado P, Mallappa S, Clark S, Thomas H, Jeffery R, Poulsom R, Rodriguez-Justo M, Novelli M, Chetty R, Silver A, Sansom OJ, Greten FR, Wang LM, East JE, Tomlinson I, Leedham SJ., Nature Medicine, 2014 December 01 --- lncRNA Directs Cooperative Epigenetic Regulation Downstream of Chemokine Signals, Xing Z, Lin A, Li C, Liang K, Wang S, Liu Y, Park PK, Qin L, Wei Y, Hawke DH, Hung MC, Lin C, Yang L., Cell, 2014 November 13 --- Expression of the Chemokine Receptor Gene, CCR8, is Associated With DUSP22 Rearrangements in Anaplastic Large Cell Lymphoma., Xing X, Flotte TJ, Law ME, Blahnik AJ, Chng WJ, Huang G, Knudson RA, Ketterling RP, Porcher JC, Ansell SM, Sidhu J, Dogan A, Feldman AL., Appl Immunohistochem Mol Morphol., 2014 November 11 --- Species Differences in Cannabinoid Receptor 2 and Receptor Responses to Cocaine Self-Administration in Mice and Rats., Zhang HY, Bi GH, Li X, Li J, Qu H, Zhang SJ, Li CY, Onaivi ES, Gardner EL, Xi ZX, Liu QR, Neuropsychopharmacology, 2014 November 06 --- Detection of immunoglobulin light chain restriction in cutaneous B-cell lymphomas by ultrasensitive bright-field mRNA in-situ hybridization, Minca EC, Wang H, Wang Z, Lanigan C, Billings SD, Luo Y, Tubbs RR, Ma XJ., J Cutan Pathol.J Cutan Pathol., 2014 November 03 --- Analysis of MET mRNA Expression in Gastric Cancers Using RNA In Situ Hybridization Assay: Its Clinical Implication and Comparison with Immunohistochemistry and Silver In Situ Hybridization., Choi J, Lee HE, Kim MA, Jang BG, Lee HS, Kim WH., PLoS One. , 2014 November 03 --- Cannabinoid CB2 receptors modulate midbrain dopamine neuronal activity and dopamine-related behavior in mice, Zhang HY, Gao M, Liu QR, Bi GH, Li X, Yang HJ, Gardner EL, Wu J, Xi ZX., Proc Natl Acad Sci U S A., 2014 November 03 ---

REVOLUTIONARY RNASCOPE® RNA IN SITU HYBRIDIZATION TECHNOLOGY

Delivers Quantitative Molecular Detection and Morphological Context (MD & MC) in a Single Assay

RNAscope® Technology is a cutting-edge multiplex nucleic acid in situ hybridization technology, based on ACD’s unique, patented probe design strategy which enables simultaneous signal amplification and background noise suppression.  RNAscope Technology represents one of the most significant advances in ISH technology in over 40 years.


What is the value of Quantitative Molecular Detection and Morphological Context (MD & MC) in a single assay? What makes RNAscope unique?

  • Molecular Detection: RNAscope detects single RNA molecules and provides quantitative analysis of gene expression at the single-cell resolution.
  • Morphological Context: RNAscope localizes RNA molecules providing spatial and cell-specific expression while preserving tissue architecture.
  • Quantitative: RNAscope quantifies RNA molecules per cell, giving accurate expression level at the single cell resolution across heterogeneous cell populations. 
  • Universal:  RNAscope's patented probe design can be applied to virtually ANY gene in ANY genome in ANY tissue.
  • Scalable: The assay is robust and easy to implement in basic, translational and clinical research labs. Availability of Manual Assays, Automated Assays and Assay Services provide ultimate flexibility.

RNAscope enables in situ detection of RNA biomarkers—filling a long standing gap in in situ molecular analysis.

RNAscope can complement IHC when antibodies are unavailable or have insufficient sensitivity or uncertain specificity. RNAscope is ideal for detecting RNAs for secreted proteins and non-coding RNA—a limitation of IHC.

Downstream of microarrays and NGS (e.g., RNA-seq), RNAscope is an ideal platform for translating new scientific advances into clinical applications due to the speed of RNAscope assay development and its superior sensitivity and specificity.

Other RNA biomarker analysis methods such as RT-PCR can provide quantitative, sensitive and specific measurements of RNA transcripts. However, the resulting quantitative gene expression information is without morphological and cellular context and only represents a “group average” of an admixture of heterogeneous populations of cells. RNAscope’s ability to provide gene expression at the single cell resolution coupled with morphological context is well-suited for RNA analysis in tissue diagnostics applications.

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