Advanced Cell Diagnostics

Provider of the most advanced
RNA in situ hybridization technology
lncRNA Directs Cooperative Epigenetic Regulation Downstream of Chemokine Signals, Xing Z, Lin A, Li C, Liang K, Wang S, Liu Y, Park PK, Qin L, Wei Y, Hawke DH, Hung MC, Lin C, Yang L., Cell, 2014 November 13 --- Expression of the Chemokine Receptor Gene, CCR8, is Associated With DUSP22 Rearrangements in Anaplastic Large Cell Lymphoma., Xing X, Flotte TJ, Law ME, Blahnik AJ, Chng WJ, Huang G, Knudson RA, Ketterling RP, Porcher JC, Ansell SM, Sidhu J, Dogan A, Feldman AL., Appl Immunohistochem Mol Morphol., 2014 November 11 --- Species Differences in Cannabinoid Receptor 2 and Receptor Responses to Cocaine Self-Administration in Mice and Rats., Zhang HY, Bi GH, Li X, Li J, Qu H, Zhang SJ, Li CY, Onaivi ES, Gardner EL, Xi ZX, Liu QR, Neuropsychopharmacology, 2014 November 06 --- Detection of immunoglobulin light chain restriction in cutaneous B-cell lymphomas by ultrasensitive bright-field mRNA in-situ hybridization, Minca EC, Wang H, Wang Z, Lanigan C, Billings SD, Luo Y, Tubbs RR, Ma XJ., J Cutan Pathol.J Cutan Pathol., 2014 November 03 --- Analysis of MET mRNA Expression in Gastric Cancers Using RNA In Situ Hybridization Assay: Its Clinical Implication and Comparison with Immunohistochemistry and Silver In Situ Hybridization., Choi J, Lee HE, Kim MA, Jang BG, Lee HS, Kim WH., PLoS One. , 2014 November 03 --- Cannabinoid CB2 receptors modulate midbrain dopamine neuronal activity and dopamine-related behavior in mice, Zhang HY, Gao M, Liu QR, Bi GH, Li X, Yang HJ, Gardner EL, Wu J, Xi ZX., Proc Natl Acad Sci U S A., 2014 November 03 --- Expression and cellular localization of the Mas receptor in the adult and developing mouse retina, Prasad T, Verma A, Li Q., Molecular Vision, 2014 October 17 --- Identification of a Novel FN1-FGFR1 Genetic Fusion as a Frequent Event in Phosphaturic Mesenchymal Tumour, Lee JC, Jeng YM, Su SY, Wu CT, Tsai KS, Lee CH, Lin CY, Carter JM, Huang JW, Chen SH, Shih SR, Mariño-Enríquez A, Chen CC, Folpe AL, Chang YL, Liang CW., J Pathol, 2014 October 13 --- Recombinant adeno-associated virus 2/5-mediated gene transfer is reduced in the aged rat midbrain, Polinski NK, Gombash SE, Manfredsson FP, Lipton JW, Kemp CJ, Cole-Strauss A, Kanaan NM, Steece-Collier K, Kuhn NC, Wohlgenant SL, Sortwell CE., Neurobiology of Aging, 2014 October 09 --- VEGF mRNA Assessment in Human Pterygium: A New 'Scope' for a Future Hope., Poenaru Sava MG, Raica ML, Cimpean AM., Ophthalmic Res. , 2014 October 03 ---


Delivers Quantitative Molecular Detection and Morphological Context (MD & MC) in a Single Assay

RNAscope® Technology is a cutting-edge multiplex nucleic acid in situ hybridization technology, based on ACD’s unique, patented probe design strategy which enables simultaneous signal amplification and background noise suppression.  RNAscope Technology represents one of the most significant advances in ISH technology in over 40 years.

What is the value of Quantitative Molecular Detection and Morphological Context (MD & MC) in a single assay? What makes RNAscope unique?

  • Molecular Detection: RNAscope detects single RNA molecules and provides quantitative analysis of gene expression at the single-cell resolution.
  • Morphological Context: RNAscope localizes RNA molecules providing spatial and cell-specific expression while preserving tissue architecture.
  • Quantitative: RNAscope quantifies RNA molecules per cell, giving accurate expression level at the single cell resolution across heterogeneous cell populations. 
  • Universal:  RNAscope's patented probe design can be applied to virtually ANY gene in ANY genome in ANY tissue.
  • Scalable: The assay is robust and easy to implement in basic, translational and clinical research labs. Availability of Manual Assays, Automated Assays and Assay Services provide ultimate flexibility.

RNAscope enables in situ detection of RNA biomarkers—filling a long standing gap in in situ molecular analysis.

RNAscope can complement IHC when antibodies are unavailable or have insufficient sensitivity or uncertain specificity. RNAscope is ideal for detecting RNAs for secreted proteins and non-coding RNA—a limitation of IHC.

Downstream of microarrays and NGS (e.g., RNA-seq), RNAscope is an ideal platform for translating new scientific advances into clinical applications due to the speed of RNAscope assay development and its superior sensitivity and specificity.

Other RNA biomarker analysis methods such as RT-PCR can provide quantitative, sensitive and specific measurements of RNA transcripts. However, the resulting quantitative gene expression information is without morphological and cellular context and only represents a “group average” of an admixture of heterogeneous populations of cells. RNAscope’s ability to provide gene expression at the single cell resolution coupled with morphological context is well-suited for RNA analysis in tissue diagnostics applications.

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